Reporter
rareGFP

Part:BBa_K1506000:Design

Designed by: Clay Swackhamer, Sam Krug, Ashlee Smith, Emily Sileo   Group: iGEM14_Penn_State   (2014-08-05)


Superfolder GFP with only rare codons


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 102
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 58
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 102
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon optimization that was used when designing this sequence was based on the codon usage profile for E. coli across its entire genome. To produce any particular amino acid, E. coli can usually use one of several codons, because of codon redundancy. Previous researchers compiled a table showing the frequency with which each codon appears in the entire genome and organized the data to show which codons are the most commonly and most rarely used to produce any given amino acid. To create this GFP, the rarest possible codon was used for each amino acid in the protein.


Source

This is a purely synthetic part.

References